Therapeutic agents from tissue cultures of medicinal plants. Strategies to improve the production of forskolin from hairy root cultures of coleus forskohlii briq. Other names include pashanabhedi, indian coleus, makandi, hl362, mao hou qiao rui hua. Coleus forskohlii in salem, tamil nadu get latest price. Uses, side effects, interactions, dosage, and warning. Chatterjee 1 and ugur cakilcioglu 2 1 ugc centre of advanced study, department of botany, university of burdwan, burdwan 7104, west bengal, india. Forskolin is a naturallyoccurring chemical that makes coleus forskohlii tick. Coleus forskohlii introduction coleus forskohlii is a botanical that has been used since ancient times in hindu and ayurvedic traditional medicine.
Solenostemon scutellarioides is an annual plant, well known and appreciated amomgst the horticulturists for the colours of its leaves fig. Plectranthus barbatus syn coleus forskohlii is an important medicinal herb belonging to the. Coleus spp micropropagation and in vitro production of secondary. Therapeutic uses, benefits and claims of coleus forskohlii. In this study, we carried out meristem culture of coleus for micropropagation. The plant profiler provides detailed information and bioactive compounds for numerous different plant species.
Coleus spp micropropagation and in vitro production of. Issn22495746 international journal of ayurvedic and. Extraction and elemental analysis of coleus forskohlii extract. Forskolin was named after the finnish botanist, forskal.
It produces forskolin, an extract useful for pharmaceutical preparations and research in cell biology. Coleus forskohliis power lies within its roots in the form of forskolin. Strategies to improve the production of forskolin from hairy. The high quality, evidencebased information on each plant profiler page is gathered by natural standard, the authority on integrative medicine. In spite of this, there have been few attempts to propagate its cultivation in india. Shoot regeneration from leaf derived callus of coleus forskohlii briq. Forskolin coleonol is a labdane diterpene that is produced by the indian coleus plant plectranthus barbatus.
Coleus tuber is rich in starch 73% on dry weight basis, but poor in. Pdf high frequency in vitro regeneration system for conservation. Further research is needed to confirm these effects, since forskolin is most often used as a research tool in vitro, or outside the body, like in a. Interaction of a medicinal plant coleus forskohlii with. Coleus forskohlii is an endangered multipurpose medicinal plant that has widespread applications. The strategies for production of forskolin visavis protection against soil borne diseases of the potential herb coleus forskohlii briq. Biotechnological approaches especially plant tissue culture are found to. Since ancient times, plants of the coleus species have been used as an herbal medicine to treat various disorders of the cardiovascular, respiratory, gastrointestinal, and central nervous systems. It is necessary to propagate the coleus because of their advantages. As obesity rates rise, scientists have focused more attention on forskolins ability to burn fat and help people lose weight. Micropropagation of coleus have been performed on several explants, namely nodal segment, shoot tip 1, binodal minitop grafting cutting 3 and leaf 4.
Plectranthus barbatus syn coleus forskohlii is an important medicinal herb belonging to the lamiaceae was selected for the present study. High frequency and rapid in vitro plant regeneration of coleus. The leaf segments were cultured on agar solidified ms media supplemented with different concentration of. The sole source of forskolin is the roots of wild or cultivated c. A high frequency shoot organogenesis and plant establishment protocol has been developed for coleus forskohlii from leaf derived callus.
Shoots were regenerated from the callus on ms medium supplemented with 4. Effect of growth regulators on in vitro organogenesis and. As with other members of the large diterpene family of natural products, forskolin is derived from geranylgeranyl pyrophosphate ggpp. Plant tissue culture plant in vitro techniques, in which plant ls, tissues and organs cel are cultivated under aseptic conditions totally independent of geographical and climatic factors, offers alternatives for producing table 1. Coleus coleus forskohlii plant profiler sigmaaldrich. A correlation between the forskolin accumulation and the growth rate was investigated using the clonally. Coleus forskohlii supplement health benefits, dosage, side. Medicinal coleus coleus forskohlii is an important medicinal crop which contains forskolin in their roots. Much of the micropropagation research on the genus coleus was. Tissue culture technique is potentially valuable for studying the biosynthesis of secondary product and may also eventually provide an efficient means of producing commercially important plant products 19. General description forskolin, present predominantly in the root of coleus forskohlii plant, is a potential pharmacological compound. The major metabolite that is found exclusively in the cork cells of the root in c.
Manoyl oxide r, the biosynthetic precursor of forskolin. The tropical genus coleus, differs from the related plectranthus by having the stamens united, but this difference is not quite consistent, thus coleus has been reduced to a synonym ryding, 1994. In vitro multiplication of coleus forskohlii briq an approach towards. Dig in to find out more health benefits of coleus forskohlii. It has become a hot plant with lots of commercial value in recent years. Rosmarinic acid production by coleus forskohlii hairy root. The adoption of plant tissue culture technology has ensured pathogenfree, diseaseresistance, and highyielding planting material in case of cf, which has helped to increase the yield, productivity, uniformity of produce, and reduced harvesting time and wastage. Transverse sections of roots diameter of approximately 15 mm were prepared for histochemical analysis. Regenerative potential in coleus forskohlii during successive subcultures. It is a most important medicinal as well as aromatic values. Effects of coleus forskohlii supplementation on body. Coleus forskohlii lamiaceae plants were grown in the greenhouse at the university of copenhagen, denmark under ambient photoperiod and 24c day17c night temperatures. Shoot organogenesis and mass propagation of coleus. The aim of this study was to characterize the root extract of the medicinal plant coleus forskohlii.
Micropropagation of coleus species micropropagation is one of the key tools of plant biotechnology that explains the totipotent nature of plant cells. Plectranthus barbatus, also known by the synonym coleus forskohlii and vernacular names forskohlii and indian coleus, is a tropical perennial plant related to the typical coleus species. Reddy, sairam p and rodrigues, rosaline and rajasekharan, ram 2001 shoot organogenesis and mass propagation of coleus forskohlii from leaf derived callus. Forskolin may help the fat cells to release free fatty acids to use.
The invention relates to a preparation method for extracting forskolin from coleus forskohlii briq the preparation method is characterized by including the steps of firstly, selecting a coleus forskohlii briq. The present communication presents an in vitro rapid regeneration method using leaf explants of coleus forskohlii through direct organogenesis. Sep 18, 2019 the main bioactive ingredient in coleus forskohlii is called forskolin. Forskolin, a labdane diterpene possesses cardiotonic, effect of growth regulators on in. Coleus forskohlii hairy root cultures were found to produce forskolin and rosmarinic acid ra as the main metabolites.
It is used for treating hypertension, respiratory disorders and obesity. Fatfighting and more by vladimir badmaev, md, phd and muhammed majeed, phd coleus forskohlii, is a member of the mint family lamiaceae. The main bioactive ingredient in coleus forskohlii is called forskolin. Forskolin has been isolated from the roots of the indiabased coleus forskohlii. Optimal callus was developed from mature leaves on. Because of continuous collection of roots from the wild sources, this plant. Positive inotropic effect of the leaf extracts of parent and. In vitro direct shoot regeneration from proximal, middle and. This plant has been used since ancient times to treat heart disorders such as high blood pressure. Coleus extract helped the body to maintain healthy weight by tending to prevent weight gain. Much of the micropropagation research on the genus coleus was concentrated on coleus forskohlii and coleus blumei. Coleus tuber is used in curry preparation, particularly for its aromatic flavor and sweetness, and also can be baked or made into chips. Coleus blumei benth, cytokinin, tissue culture, multiple shoots. Forskolin 7 betaacetoxy8, epoxy1 alpha,6 beta,9 alphatrihydroxylabd14ene11one is the main active ingredient in the ayurvedic herb coleus forskohlii.
The effective range of the competitive enzymelinked immunosorbent assay elisa test for detection of forskolin content in clonally propagated plant organs of coleus forskohlii using monoclonal antibodies extends from 5ng to 5mcg. The company ensures the transit safety as well as timely shipments of the coleus forskohlii plant, with the pricing being reasonable. It grows at 6001800 m elevation on sunexposed hill slopes. Through forskolin, coleus forskohlii supplementation may increase testosterone, and protect against cancer and inflammation. Tissue explants of an important indian medicinal plant, coleus forskohlii were taken. The plant is native to subtropical and tropical regions of india and east africa, grows to a height of 60 cm, and has large, soft leaves. Cn106349206a preparation method for extracting forskolin. Shoot organogenesis and mass propagation of coleus forskohlii. Synonyms include plectranthus forskalaei, plectranthus forskohlii, plectranthus kilimandschari, plectranthus grandis, coleus forskohlii, coleus barbatus, and coleus coerulescens. Coleus tuber is rich in starch 73% on dry weight basis, but poor in protein content 7% on dry weight basis. The leaf and root extracts can be utilized for the treatment of various ailments like bronchitis, asthma, hay fever, angina and abdominal disorders.
Chatterjee 1 and ugur cakilcioglu 2 1 ugc centre of advanced study, department of botany, university of. Propagation through tissue forskolin culture in mother plant. Plectranthus barbatus is a popular medicinal plant belonging to the family lamiaceae and order lamiales. The tuberous roots of coleus forskohlii a rich source of forskolin coleonol. The root portion of the plant has been traditionally used for medicinal purposes and contains the active constituent, forskolin. Although many medicinal plants have been propagated using plant tissue cultures, literature pertaining to the evaluation of the pharmacological activity of these extracts is rather limited.
It is also called as hausa potato, chinese potato, country potato, or coleus potato. Coleus is a not a particularly remarkable plant to look at, yet it has a 3000year history in ayurvedic medicine and is mentioned in ancient sanskrit texts as a tonic for a healthy heart and lungs. Gelatin, microcrystalline cellulose plant fiber, may contain one or both of the following. Forskolin is a chemical found in the roots of the plant plectranthus barbatus coleus forskohlii. The strategies for production of forskolin visavis. Plant biotechnology laboratory, department of botany. Forskolin, a labdane diterpene possesses cardiotonic, effect of growth regulators on in vitro organogenesis. Forskolin coleus forskohlii coleus and hypothyroidism forskolin has demonstrated the ability to increase thyroid hormone production and stimulate thyroid hormone release. Issn22495746 international journal of ayurvedic and herbal. It is an endangered plant so it is necessary for the plant tissue culture of coleus forskohlii. Bioactive secondary metabolites from plant tissue cultures. May 01, 2009 the thymol content in the parent plant was 0. Even the recent studies and researches conducted to confirm the medicinal properties of coleus showed that the forskolin extract from the plant has numerous health benefits including anti hypertensive and weightloss properties.
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